![]() Video: Replication of RNA Viruses This lesson will give you a basic overview of how different types of RNA viruses replicate inside of an animal cell. We will cover the essence of being double-stranded and retroviruses, as well as positive-and negative-sense RNA viruses. Yuri Malakhov Answers Questions about Parkhomov Replication (Video) Posted on January 26, 2016 • 119 Comments This video below features Russian Researcher Yuri Malakhov answering questions about replication using both Ni and LAH from which he received from Alexander Parkhomov. Replication by the type of rolling ring leads to the formation of multiple copies of the original ring molecule of the nucleic acid. DNA replication by rolling ring type is initiated by a special -, encoded DNA plasmid or bacteriophage, which breaks one of the chains of the double-stranded ring molecule in the DSO site (double-strand origin). The initiator protein binds to the 5 'end of the broken chain, and the free 3'-serves for DNA. Using an unbroken chain as a template, the entire circular DNA molecule undergoes replication, replacing the broken chain representing single-stranded DNA. The replacement of the broken chain is performed by the encoded PcrA recipient in the presence of the plasmid-encoded initiating replication factor. Prolonged DNA synthesis leads to the formation of multiple single-stranded linear copies of the original DNA in the form of long DNA sequences called. Linear copies can go into a double-stranded ring molecule through the following process. Initially, the initiator protein produces another break, terminating thereby the synthesis of the other (leading) chain. Then, single-stranded origin is replicated in a single-stranded SSO-DNA, forming a new double-stranded ring. After that, DNA polymerase I replaces the primer with DNA, and cross-links the ends, thereby forming the final double-stranded DNA. Replication by the rolling ring type has found wide application both in academic studies and in, and this mechanism has been successfully used for DNA from very small amounts of DNA from the original sample. Virology [| ] Some viruses replicate their DNA in the host cell by replicating like a rolling ring. For example, (HHV-6) expresses a series of 'early genes' that are believed to be involved in this process. The long concatemers formed during this replication are separated by ribozymes between the regions pac-1 and pac-2 HHV-6 during the formation of individual. The video is licensed under the Creative Commons license and is broadcasted from www.academicearth.org. Molecular biology of prokaryotes and eukaryotes and their viruses. Mechanisms of DNA replication, transcription and translation. Structure of genes and chromosomes. Regulation of gene expression. Biochemical processes in the membrane, membrane structure and functions, intercellular interaction, intracellular structure, cell skeleton, nuclear-cytoplasmic transport, signal transduction mechanism, control of the cell cycle.
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Principles of Russian Entrepreneurial Law Business law, like any other branch of Russian law, is based on certain principles that characterize and determine the legal regulation in the field of business law. The principles of Russian business law are the fundamental principles on which business law is built. There are a number of principles of business law. The principle of freedom of entrepreneurial activity is fixed in Art. 8, 34 of the Constitution of the Russian Federation, which states: 'Everyone has the right to freely use his abilities and property for entrepreneurial and other economic activities not prohibited by law.' Therefore, each citizen decides independently, engage in entrepreneurial activities or not, what organizational and legal form and type of entrepreneurial activity to elect, etc. This principle is developed in the Civil Code and other regulatory legal acts. 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